Crystal structure of glutathione-dependent dehydroascorbate reductase from spinach chloroplast
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چکیده
منابع مشابه
Purification and characterization of glutathione-dependent dehydroascorbate reductase from rat liver.
GSH-dependent enzymic reduction of dehydroascorbic acid to ascorbic acid has been studied in rat liver cytosol. After gel filtration of cytosol on Sephadex G-100 SF, dehydroascorbate reductase activity was recovered in two distinct peaks, one corresponding to glutaredoxin (an enzyme already known for its dehydroascorbate reductase activity) and another, much larger one, corresponding to a novel...
متن کاملTobacco chloroplast transformants expressing genes encoding dehydroascorbate reductase, glutathione reductase, and glutathione-S-transferase, exhibit altered anti-oxidant metabolism and improved abiotic stress tolerance.
One approach to understanding the Reactive Oxygen Species (ROS)-scavenging systems in plant stress tolerance is to manipulate the levels of antioxidant enzyme activities. In this study, we expressed in the chloroplast three such enzymes: dehydroascorbate reductase (DHAR), glutathione-S-transferase (GST) and glutathione reductase (GR). Homoplasmic chloroplast transformants containing either DHAR...
متن کاملPea chloroplast glutathione reductase: purification and characterization.
Glutathione reductase (EC 1.6.4.2) was purified from intact pea (Pisum sativum) chloroplasts by a method which includes affinity chromatography on ADP-agarose. Fractions from the affinity column which had glutathione reductase activity consisted of polypeptides of 60 and 32 kilodaltons. Separation of the proteins by electrophoresis on native gels showed that glutathione reductase activity was a...
متن کاملEffect of polyamines on glutathione reductase activity in spinach.
The effects of polyamines (putrescine, spermidine and spermine) on glutathione reductase (glutathione: NADP+ oxidoreductase, EC 1.8.1.7; GR) activity of spinach leaves (Spinacia oleracea L. cv. Gladiator) were investigated under in vivo and in vitro conditions. Spinach was grown in sand culture under controlled conditions for 30 d. In in vivo assays 30-day-old plants were sprayed with polyamine...
متن کاملCrystal Structure of Biliverdin Reductase
A heme (Fe-porphyrin complex) is a prosthetic group of hemoproteins such as hemoglobin, myoglobin, cytochromes and so on. In biological systems, the heme is decomposed by two enzymes (hemeoxygenase and biliverdin reductase) and excreted (Fig. 1). Biliverdin reductase (BVR) catalyzes the last step of the heme catabolism, in which the biliverdin is reduced into bilirubin with two electrons from N...
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ژورنال
عنوان ژورنال: Acta Crystallographica Section A Foundations of Crystallography
سال: 2005
ISSN: 0108-7673
DOI: 10.1107/s0108767305091348